产品说明
应用
Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay. It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.
生化/生理作用
Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage. It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.
包装
Packaging
50, 500 g in poly bottle
分析说明
以下是与我们的琼脂糖相关的属性列表：
硫酸盐含量 - 用作一种纯度的指标，因为硫酸盐是存在的主要离子基团。
凝胶强度 - 施加在凝胶上让其破裂所必需的力。
凝胶点 - 琼脂糖水溶液在冷却时形成凝胶的温度。 琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说，它们的凝胶点与它们的熔点温度不同。
电内渗(EEO)- 液体通过凝胶的一种运动。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动，但可解离的抗衡阳离子可以向基质中的阴极迁移，从而产生EEO。 由于生物聚合物的电泳运动通常是朝向阳极的，因此EEO会因为内部的对流而破坏分离。
基本信息
| EC 号 | 232-731-8 |
| MDL编号 | MFCD00081294 |
| PubChem化学物质编号 | 24845663 |
| NACRES | NA.25 |
产品性质
| 质量水平 | 100 |
| 等级 | for molecular biology |
| 形式 | powder |
| 杂质 | DNases, none detected RNases, none detected phosphatases, none detected proteases, none detected |
| 灼烧残渣 | ≤1% |
| 缺失 | ≤10% loss on drying |
| EEO | 0.23-0.27 |
| 转变温度 | gel point 34-37 ℃ (1.5% solution) |
| 凝胶强度 | ≥1500 g/cm2 |
| 痕量阴离子 | chloride (Cl-): ≤0.3% sulfate (SO42-): ≤0.6% |
| 痕量阳离子 | Ca: ≤0.05% Cd: ≤0.001% Co: ≤0.001% Cr: ≤0.001% Cu: ≤0.001% Fe: ≤0.001% K: ≤0.05% Mg: ≤0.001% Mn: ≤0.001% Na: ≤0.5% Ni: ≤0.001% Pb: ≤0.001% Zn: ≤0.001% |
| InChI | 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1 |
| InChI key | MJQHZNBUODTQTK-WKGBVCLCSA-N |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
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