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CypExpressTM 1A2 Cytochrome P450 human

品牌
Sigma-Aldrich
货号
MTOXCE1A2
包装型号
规格纯度
参考价格
3450.58 *本价格含增值税费
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服务
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数量
-+
产品名称:
CypExpressTM 1A2 Cytochrome P450 human
CP12
P450(PA)
CYP1A2
P450 1A2
P3-450
产品介绍:

产品说明

应用

Poster: A Novel, Robust Recombinant Human P450 Biocatalytic System

Poster: Evaluation of Dry Powder Preparations of Permeabilized
Pichia pastoris Cells Expressing Human Cytochrome P450 2D6 Enzyme (CYP2D6) for Drug Metabolite Generation

Poster: Facile Scale-up Biocatalytic Production of P-450 Metabolites Using a Novel Recombinant Human Catalytic System

Poster: Multi-Cycle Production of Metabolites with human P450 systems

Poster: Rapid identifcation and production of metabolites using CypExpress 2D6m 3A4 and 2C9

Poster: Rapid identifcation and production of metabolites using stabilized dried powder (SDP) of human cytochrome P450S (hCYPS) 2D6, 3A4, and 2C9 engineered in yeast

Poster: Utilizing a Novel Biocatalytic Human P450 System for the Multi - Cycle Production of Metabolites

生化/生理作用

Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. Furafylline and α-naphthoflavone are inhibitors, whereas omeprazole and lansoprazole are inducers of the enzyme. The isozyme CYP1A2 is a major pathway for the in vivo conversion of phenacetin into acetaminophen via O-deethylation. This isozyme also catalyzes the 5-hydroxylation of thiabendazole.
Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous.

特点和优势

CypExpress 1A2 is a permeabilized and stabilized dried yeast powder preparation containing full length, unmodified, human CYP 1A2 and recombinant human NADPH oxidoreductase. Using proprietary processes, CypExpress are expressed in Pichia yeast. Following optimal expression, the cells are inactivated, permeabilized and dried to a fine powder. CypExpress retains the cellular mechanisms to provide the P450 enzymes with the energy and cofactors to continue to function for long experiments, and can generate larger amounts of metabolite than mammalian microsomes or other genetically engineered expression systems. It is designed for rapid screening of drug metabolites and for scaling up metabolite generation.

单位定义

A reaction mixture containing 500 μM phenacetin and 100 mg/mL of CypExpress 1A2 will produce 35 μM acetaminophen in 4 hours at 30℃ under the conditions described.

外形

Light yellow, very fine powder

重悬

Per Protocol/Variable

法律信息

6538 is a trademark of American Type Culture Collection
CypExpress is a trademark of Oxford Biomedical Research

基本信息

EC 号232-387-9
NACRESNA.77

产品性质

生物来源human
重组expressed in Pichia pastoris
形式dry powder
UniProt登记号P05177
application(s)cell analysis
运输dry ice
储存温度−70℃
Gene Informationhuman ... CYP1A2(1544)

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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