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Bolton Broth Selective Supplement

品牌
Millipore
货号
1.00079
规格纯度
according to ISO 10272-1, for Campylobacter spp., pkg of 10 vials, For the preparation of Bolton Selective Enrichment Broth
参考价格
4161.64 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Bolton Broth Selective Supplement
Campylobacter Selective Supplement
产品介绍:

产品说明

一般描述

Bolton Broth Selective Supplement is a mixture of four different antibiotics in lyophilized form. Vancomycin, Cefoperazone, and Trimethoprim inhibit the growth of Gram-positive and Gram-negative bacteria. Amphotericin B largely reduces the growth of Yeasts and Molds.

应用

Bolton Broth Selective Supplement is an additive used during the preparation of Bolton Selective Enrichment Broth for the enrichment of Campylobacter from foods.

联系

Replaces: 1.00079.0017

分析说明

Growth promotion test according to ISO 11133
Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
Additionaly monoculture of E.coli and P.mirabilis.
Afterwards confirmation on mCCDA resp. Tryptic soy agar
Inoculum on reference medium
- Campylobacter coli ATCC 43478 (WDCM 00004):
10 - 100
- Campylobacter jejuni ATCC 33291 (WDCM 00005): 10 - 100
- Campylobacter jejuni ATCC 29428 (WDCM 00156): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Proteus mirabilis ATCC 29906 (WDCM 00023): ≥ 1000
Growth on mCCDA
- Campylobacter coli ATCC 43478 (WDCM 00004):
>10 CFU
- Campylobacter jejuni ATCC 33291 (WDCM 00005): >10 CFU
- Campylobacter jejuni ATCC 29428 (WDCM 00156): >10 CFU
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Proteus mirabilis ATCC 29906 (WDCM 00023): no growth
Colony colour
- Campylobacter coli ATCC 43478 (WDCM 00004):
greyish
- Campylobacter jejuni ATCC 33291 (WDCM 00005): greyish
- Campylobacter jejuni ATCC 29428 (WDCM 00156): greyish
Incubation:
Bolton broth: 5 hours at 44°C, afterwards 44 hours at 41.5°C, Anaerocult C
Confirmation on mCCDA: at least 44 hours at 41.5°C, Anaerocult C
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

产品性质

质量水平200
agencyaccording to ISO 10272-1
reg. complianceFDA -BAM
形式lyophilized powder
组成Amphotericin B, 5 mg
Cefoperazone, 10 mg
Trimethoprim, 10 mg
Vancomycin, 10 mg
包装pkg of 10 vials
technique(s)microbiological culture: suitable
application(s)food and beverages
microbiology
pathogen testing
储存温度2-8℃
适用性Campylobacter spp.
Listeria spp.

安全信息

象形图GHS08
警示用语:Danger
危险声明H317 - H334 - H361d - H373
预防措施声明P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
危险分类Repr. 2 - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 2
储存分类代码11 - Combustible Solids
WGKWGK 3

Sigma-Aldrich

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