产品说明
一般描述
A quenched fluorescent substrate for MMPs (excitation maximum: ~325 nm; emission maximum: ~393 nm). MMP-3 (stromelysin-1) hydrolyzes this substrate (kcat/Km = 59,400 M-1s-1) at a rate 60X faster than hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; kcat/Km = 54,000 M-1s-1), and MMP-9 (gelatinase-B; kcat/Km = 55,300 M-1s-1).
A quenched fluorescence substrate for MMPs. MMP-3 (stromelysin-1) hydrolyzes this substrate (kcat/Km = 5.9 x 104 M-1sec-1) at a rate 60 times faster than the hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; kcat/Km = 5 x 104 M-1s-1), and MMP-9 (gelatinase-B; kcat/Km = 5.5 x 104 M-1s-1).
包装
1 mg in Plastic ampoule
生化/生理作用
kcat/Km = 5.9 x 104 M-1sec-1 as substrates for MMP-3 (stromelysin-1)
Product does not compete with ATP.
Primary Target
Matrix Metalloproteinases (MMPs)
Reversible: no
Cell permeable: no
警告
Toxicity: Standard Handling (A)
序列
MCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)
外形
Supplied as a trifluoroacetate salt.
重悬
Following initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
其他说明
Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C79H105N19O19S |
分子量 | 1656.86 |
产品性质
质量水平 | 100 |
测定 | ≥98% (HPLC) |
形式 | lyophilized solid |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze desiccated protect from light |
颜色 | yellow |
溶解性 | DMSO: 5 mg/mL |
荧光 | λex ~325 nm λem ~393 nm |
运输 | ambient |
储存温度 | −20℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich