产品说明
一般描述
Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
This PhosphoDetect
Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
免疫原
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
Human
包装
1 set in Plastic ampoule
应用
ELISA (0.05 µg/ml)
Immunoblotting (0.5 µg/ml)
警告
Toxicity: Standard Handling (A)
外形
100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
重悬
Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
分析说明
Positive Control
Pervanadate-treated HepG2 (included)
其他说明
For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 2D3, monoclonal |
形式 | liquid |
包含 | ≤0.1% sodium azide as preservative (antibody only) |
species reactivity | mouse, human |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze |
同位素/亚型 | IgG1 |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich