产品说明
一般描述
Recombinant, human topoisomerase I fused to a His•Tag
Recombinant, human topoisomerase I fused to a His•Tag
包装
1 set in Glass bottle
Please refer to vial label for lot-specific concentration.
生化/生理作用
0.1-1 unit (ng) is sufficient for relaxing 0.5 µg of pBR322 supercoiled DNA in a 20 µl reaction.
警告
Toxicity: Standard Handling (A)
外形
5000 U enzyme in 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9 and 1 vial dilution buffer (100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0).
重悬
Following initial thaw aliquot and freeze (-70°C). For storage of diluted protein, dilute in storage buffer with 500µg/ml molecular biology grade BSA, aliquot, and freeze (-70°C).
其他说明
Pommier, Y., et al. 1998. Biochim. Biophys. Acta1400, 83.
Pourquier, P., et al. 1997. J. Biol. Chem.272, 26441.
Rossi, F., et al. 1996. Nature381, 80.
Sekiguchi, J., et al. 1996. Proc. Natl. Acad. Sci. USA93, 785.
Giovanella, B.C., et al. 1989. Science246, 1046.
Wang, J,C. 1985. Annu. Rev. Biochem.54, 665.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
测定 | ≥95% (SDS-PAGE) |
质量水平 | 100 |
形式 | liquid |
specific activity | 1 units/ng protein |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
运输 | wet ice |
储存温度 | −70℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
Sigma-Aldrich