产品说明
一般描述
Recognizes recombinant and monocyte-derived TNF-α. Exhibits some cross-reactivity with lymphotoxin (TNF-β) and mouse TNF-α in L929 cells.
Rabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes recombinant and monocyte-derived TNF-α.
This Anti-TNF-α Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Neutralization Studies, Paraffin Sections, Radioimmunoassay for the detection of TNF-α.
免疫原
recombinant, human TNF-α
包装
1 mg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
ELISA (see comments)
Frozen Sections (see comments)
Immunoblotting (see comments)
Neutralization Studies (see comments)
Paraffin Sections (see comments)
Radioimmunoassay (see comments)
警告
Toxicity: Standard Handling (A)
单位定义
1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)
分析说明
Positive Control
L929 cells
其他说明
Reacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | liquid |
不包含 | preservative |
species reactivity | human, mouse (weakly) |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
杂质 | ≤1 EU/mg Endotoxins |
同位素/亚型 | IgG |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich