产品说明
应用
PhotoCol™-RUT bioink kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents. The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the α-amines groups on the N-termini. Over 20% of the total lysine residues of the collagen molecule have been methacrylated. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of ruthenium and sodium persulfate to be formulated in 1X cell culture media or PBS, which allows visible light photocrosslinking of the printed structure at 400-450 nm. PhotoCol™-RUT provides native-like 3D collagen gels, and the final gel stiffness can be customized by changing collagen concentrations and crosslinking.
法律信息
PhotoCol is a trademark of Advanced BioMatrix, Inc.
产品性质
描述 | Methacrylated collagen: Degree of methacrylation ≥ 20% Product components : Methacrylated collagen (100 mg) 20 mM acetic acid (50 mL) Neutralization solution (10 mL) Ruthenium (100 mg) Sodium persulfate photoinitiator (500 mg) |
质量水平 | 100 |
无菌性 | sterile; sterile-filtered |
杂质 | ≤10 EU/mL Endotoxin |
application(s) | 3D bioprinting |
储存温度 | 2-8℃ |
安全信息
象形图 | |
危险声明 | H411 |
预防措施声明 | P273 - P391 - P501 |
危险分类 | Aquatic Chronic 2 |
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
Sigma-Aldrich