产品说明

一般描述

The REDExtract-N-Amp^™ Plant PCR Kits enable the rapid detection of plant pathogens by direct PCR (polymerase chain reaction). These kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by direct PCR. The REDExtract-N-Amp PCR ReadyMix^™ contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

应用

REDExtract-N-Amp^™ Plant PCR Kit has been used to:

• extract genomic DNA from mycelial lawns and leaves
• screen leaf tissues from putative transgenic potato plants
• perform polymerase chain reaction (PCR)

特点和优势

• Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
• No freezing, mechanical disruption, organic extraction, column purification or precipitation required
• Specially formulated PCR ReadyMix for use with extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA
• REDExtract-N-Amp^™ requires no loading buffers or tracking dyes required for gel analysis
• Compatible with high-throughput requirements for genetic analysis of plants
• Extract stable at 4 ℃ for at least 6 months

组分

试剂盒含有稀释溶液、提取溶液和REDExtract-N-Amp PCR Reaction Mix。

其他说明

更多信息，请见www.sigma-aldrich.com/extract-n-amp。

法律信息

凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权(如，5′ Nuclease Process专利权)。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
REDExtract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC

基本信息

产品性质

安全信息