N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)

产品名称：

7365-45-9
HEPES
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
羟乙基哌嗪乙硫磺酸
4-(2-羟乙基)哌嗪-1-乙磺酸
N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)

产品介绍：

产品说明

一般描述

HEPES has been described as one of the best all-purpose buffers available for biological research.[7] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[1]

应用

HEPES has been used:

As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo†
As a component of Hanks′ solution used in the degradation testing of Iron†
As a component of HEPES medium in the transfection of cell culture with plasmids†
Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells†
As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies†

基本信息

经验(实验)分子式	C₈H₁₈N₂O₄S
分子量	238.30
Beilstein	883043
MDL编号	MFCD00006158

产品性质

质量水平	200
生物来源	synthetic
等级	reagent grade
测定	≥99% (titration)
形式	crystalline
有效pH范围	6.8 - 8.2
pKa (25 ℃)	7.5
SMILES string	OCCN1CCN(CC1)CCS(O)(=O)=O
InChI	1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key	JKMHFZQWWAIEOD-UHFFFAOYSA-N

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable