产品说明
细胞系来源
Horse sub-mandibular lymph node x AFRC 17D1
细胞系描述
The heterohybridoma AFRC 84C6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 84C6 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).
免疫原
Influenza A equine 2 virus
应用
Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.
培养基
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37℃
其他说明
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产品性质
生物来源 | Horse x Mouse/Horse Hybridoma |
ImmunoDon | Horse sub-mandibular lymph node |
骨髓瘤 | AFRC 17D1 |
反应性 | Anti-Haemagglutinin, influenza |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | −196℃ |
Sigma-Aldrich