产品说明

一般描述

The REDExtract-N-Amp^™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR (polymerase chain reaction). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix^™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

应用

REDExtract-N-Amp^™ Blood PCR Kit has been used to extract genomic DNA from blood samples, peripheral blood leukocytes, parasites and endocervical swab eluates of mice. It has also been used to perform polymerase chain reaction (PCR) using oligonucleotide primers.

特点和优势

• Efficient 8 minute prep allows greater speed and throughput
• No need for organic extraction, centrifugation or alcohol precipitation
• Hot start antibody for highly specific PCR amplification of genomic DNA
• Can be used with whole blood or blood cards
• Extract stable at 4 ℃ for at least 6 months
• Compatible with any format (single tube, 96-well, etc.)
• Simple, 3 step procedure with no special equipment required
• REDExtract-N-Amp^™ requires no loading buffers or tracking dyes required for gel analysis
• PCR products are suitable for direct cloning and sequencing

原理

只需加入Extraction Solution并在室温下孵育5分钟，即可从10 μl全血中提取出基因组DNA。向提取物中加入Neutralization Solution，以便在PCR反应前中和抑制物。随后选取一部分DNA提取物，加入专门配制的PCR ReadyMix。

其他说明

更多信息，请见www.sigma-aldrich.com/extract-n-amp。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权(如，5′ Nuclease Process专利权)。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
REDExtract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC

基本信息

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H314
预防措施声明	P280 - P305 + P351 + P338 - P310
危险分类	Skin Corr. 1B
储存分类代码	8B - Non-combustible, corrosive hazardous materials