Immunoprecipitation Vector Kit

产品说明

一般描述

The Immunoprecipitation (IP) Vector Kit provides a unique method to validate protein-protein interactions in a convenient 96-well format. This system enables potential binding partners for the protein of interest to be tagged with ”FLAG and c-Myc epitopes, followed by capture and detection using the ANTI-FLAG M2 High Sensitivity Capture Plate.

The kit provides expression vectors ”pFLAG-CMV”-2 and pc-Myc-CMV-2 designed for the cloning and expression of protein interaction candidates as N-terminal FLAG and N-terminal c-Myc fusions. Control plasmids pFLAG-CMV-2-p53, pC-Myc-CMV-2-Large T antigen and pC-Myc-CMV-2-BAP supplied are for expression of positive and negative binding partners in the IP analysis. The kit also includes the MF-2 and MR-2 verification primers.

Vector Maps and Sequences

特点和优势

• Fast and convenient 96 well plate format
• Cleaner analysis due to strongly bound antibody
• Rapid, sensitive, quantitative results

组分

The IP Vector Kit contains:

• pFLAG-CMV-2 Expression Vector (10 μg)
• pc-Myc-CMV-2 Expression Vector (10 μg)
• pFLAG-CMV-2-p53 control plasmid (20 μg)
• pc-Myc-CMV-2-Large T antigen control plasmid (10 μg)
• pc-Myc-CMV-2-BAP control plasmid (10 μg)
• Verification Primer-MF (2 nmol)
• Verification Primer-MR (2 nmol)

基本信息

产品性质

组份列表

组份不可单独销售

安全信息