产品说明
一般描述
SYBR® Green Extract-N-Amp™ Plant PCR Kit is recommended for fast and reliable direct PCR from a wide range of plant leaf tissue.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
特点和优势
其他说明
该实验方案采用20 μl规格的PCR反应。
法律信息
Extract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
基本信息
NACRES | NA.55 |
产品性质
质量水平 | 200 |
用途 | sufficient for 100 preparations sufficient for 100 reactions |
特点 | dNTPs included hotstart |
technique(s) | qPCR: suitable |
detection method | SYBR® Green |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H315 - H317 - H319 - H411 |
预防措施声明 | P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 |
危险分类 | Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 |
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |