UKKi007-A

产品说明

一般描述

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

细胞系来源

Depositor
Klinikum der Universität zu Köln

细胞系描述

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Berlin, Germany
Tissue collection date: June 15, 2009
Passage number reprogrammed: P3
Reprogramming method
Vector type: Integrating
Vector: Virus
Virus type: Retrovirus
Have the reprogramming vectors been silenced: Yes
Merthods used: rtpcr
Notes on reprogramming vector silencing: Retrovirally encoded reprogramming factors OCT4, KLF4 and cMYC are not expressed as determined by RT-PCR. Only Sox2 seems to be expressed. at very low levels.
Files showing reprogramming vector expressed or silenced: Image / Result
Vector map: File
Characterization
Analysis of Undifferentiated Cells
Marker expression: TRA 1-80(+)SSEA-4 (+)POU5F1 (OCT-4) (+)Alkaline Phosphatase(+)NANOG(+)
Differentiation potency
Ectoderm: In vivo teratoma
Endoderm: In vivo teratoma
Mesoderm: In vivo teratoma
In vitro spontaneous differentiation
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Not done
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation
Genotyping
Karyotyping
Passage number: 34
Cell line karyotype: 46, XX
Karyotyping method: Molecular karyotyping by SNP array
Genotyping
Genome-wide analysis:
SNP genotyping using OmniExpress Exome Chip
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

联系

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

传代培养常规

Medium: Essential E8^®
Passage method: EDTA
Matrix: Vitronectin
CO₂ concentration: 5%
O₂ concentration: 21%
Temperature: 37℃

法律信息

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft

产品性质

安全信息