产品说明
一般描述
Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GG-6 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified human IgG (Fab fragment). IgGs are the most common Immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid and a key players in the humoral immune response. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), they consist of a variable Fab fragment (which includes the antibody recognition site), and a conserved Fc fragment. The IgG subclasses differ in their physical and chemical properties, their distribution pattern is found to be age-dependent, and every subclass has a specific biological function.
Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GG-6 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified human IgG.
免疫原
Purified human IgG (Fab Fragment)
应用
Direct ELISA: a working dilution of 1:16,000-1:32,000 is recommended using 2.5 μg/mL human IgG for coating.
生化/生理作用
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants. IgG deficiencies are often associated with various diseases.
外形
Supplied as a lyophilized powder.
其他说明
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
基本信息
NACRES | NA.46 |
产品性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | peroxidase conjugate |
抗体形式 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
克隆 | GG-6, monoclonal |
形式 | lyophilized powder |
species reactivity | human |
浓度 | ~2 mg/mL |
technique(s) | direct ELISA: 1:16,000-1:32,000 using 2.5 μg/ml human IgG for coating. |
同位素/亚型 | IgG1 |
运输 | dry ice |
储存温度 | −20℃ |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H317 |
预防措施声明 | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
危险分类 | Skin Sens. 1 |
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich