Anti-phospho-NFAT1 (pSer⁵⁴) antibody produced in rabbit

产品说明

一般描述

Nuclear factor of activated T cells (NFAT) is a family of transcription factors implicated in multiple biological processes including cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. NFAT1 (also known as NFATc2 or NFATp) is a 154 kDa member of this family that is regulated by the calcium-dependent phosphatase calcineurin. When calcineurin is activated by calcium it dephosphorylates multiple residues in the regulatory domain of NFAT1, leading to its translocation to the nucleus and activation of its transcriptional activity. Once in the nucleus, NFAT proteins act synergistically with the AP-1 transcription factor complex to regulate the expression of multiple genes. Serine 54 in mouse NFAT1 has been shown to be important in the regulation of its transcriptional activity.
Rabbit polyclonal anti-phospho-NFAT1 (pSer⁵⁴) antibody detects mouse NFAT1. Other species have not been tested.

免疫原

synthetic phosphopeptide corresponding to a region of mouse NFAT1 that contains serine 54.

应用

Rabbit polyclonal anti-phospho-NFAT1 (pSer54) antibody is used to tag phospho-NFAT1 (pSer54) for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunoprecipitation, and immunofluorescence. It is used as a probe to determine the presence and roles of phospho-NFAT1 (pSer54) in the regulation of transcription by NFAT1.

外形

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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