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Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal

Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
A1949
规格纯度
clone RG-96, purified from hybridoma cell culture
参考价格
4226.03 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal
Monoclonal Anti-Rabbit IgG (γ-chain specific)
产品介绍:

产品说明

一般描述

The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.
Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.

免疫原

Rabbit IgG

应用

Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in

  • various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
  • westernblotting
  • immunohistology
  • dot immunobinding assay

外形

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

制备说明

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.46

产品性质

质量水平200
生物来源mouse
偶联物peroxidase conjugate
抗体形式purified immunoglobulin
antibody product typesecondary antibodies
克隆RG-96, monoclonal
形式lyophilized powder
species reactivityrabbit
should not react with chicken, guinea pig, goat, horse, sheep, rat, bovine, human, pig, feline, canine
包装vial of 0.5 mL
technique(s)direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)
同位素/亚型IgG1
储存温度2-8℃

安全信息

象形图GHS07
警示用语:Warning
危险声明H317
预防措施声明P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
危险分类Skin Sens. 1
储存分类代码13 - Non Combustible Solids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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