您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Monoclonal Anti-Phosphotyrosine antibody produced in mouse

Monoclonal Anti-Phosphotyrosine antibody produced in mouse

品牌
Sigma-Aldrich
货号
P4110
规格纯度
clone PY20, purified immunoglobulin, buffered aqueous glycerol solution
参考价格
3598.85 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Monoclonal Anti-Phosphotyrosine antibody produced in mouse
Phospho-Tyr
p-Tyr
Phospho-tyrosine
Monoclonal Anti-Phosphotyrosine
产品介绍:

产品说明

一般描述

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediate numerous signalling pathways in both prokaryotic and Eukaryotic cells. Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Tyrosine-specific protein kinase activity has also been described in many retroviral oncogene proteins. Cells transformed by these oncogenes contain elevated levels of phosphotyrosine. Many of the oncogenes found in mammalian oncogenic viruses encode tyrosine protein kinases that reside in the cellular cytoplasm. Others encode transmembrane receptors whose tyrosine phosphotransferase activity is stimulated by the binding of ligand to the extracellular domain.
Monoclonal Anti-Phosphotyrosine is specific for both native and denatured proteins containing phosphorylated tyrosine.
Protein phosphorylation is a basic mechanism for the modification of protein function in eukaryotic cells. Tyrosine phosphorylation is a rare post-translational event in normal tissue, accounting for only 0.03% of phosphorylated amino acids. The level of phosphorylated tyrosine in many cellular proteins increases tenfold following various activation processes that are mediated through phosphotyrosine kinases. The importance of tyrosine phosphorylation has been established by the demonstration that it is an integral response in many different mitogenic receptor systems.
Mouse monoclonal clone PY20 anti-Phosphotyrosine is specific for both native and denatured proteins containing phosphorylated tyrosine. Antibody binding is inhibited with phosphotyrosine and phenylphosphate, but not phosphoeserine or phosphothreonine.

免疫原

phosphotyrosine-protein conjugate

应用

Monoclonal anti-phosphotyrosine antibody produced in mouse has been used in the detection of tyrosine phosphorylation in proteins by 2D western blotting and western blotting.

外形

Solution in 20 mM phosphate buffered saline, pH 7.5, containing 50% glycerol and 3 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.44

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆PY20, monoclonal
形式buffered aqueous glycerol solution
technique(s)immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG2b
运输wet ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品