产品说明
一般描述
Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).
应用
Carboxy-terminal FLAG-BAP™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).
生化/生理作用
The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.
其他说明
Control protein
外形
Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2
制备说明
Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml
法律信息
FLAG-BAP is a trademark of sigma-aldrich Co. LLC
基本信息
| MDL编号 | MFCD00130921 |
| NACRES | NA.32 |
产品性质
| 形式 | liquid |
| 质量水平 | 200 |
| 分子量 | ~49 kDa |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
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