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D-2-Hydroxyglutarate (D2HG) Assay Kit

品牌
Sigma-Aldrich
货号
MAK320
规格纯度
sufficient for 200 fluorometric reactions
参考价格
8648.87 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
D-2-Hydroxyglutarate (D2HG) Assay Kit
产品介绍:

产品说明

一般描述

The level of D-2-Hydroxyglutarate (D2HG) is low in normal cells and tissues, but is significantly elevated in metabolic diseases, such as the rare autosomal disorder D2HG aciduria. D2HG is mildly elevated in other metabolic disorders including multiple acyl-CoA dehydrogenase deficiency, dihydrolipoyl dehydrogenase deficiency, pyruvate decarboxylase deficiency and pyruvate carboxylase deficiency, various cancers, and in neoplasms with mutations in the isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) genes. Detection of elevated D2HG is an important biomarker for early diagnosis, prognosis, monitoring and the development of therapeutic strategies against these diseases.

特点和优势

  • Highly sensitive and rapid enzymatic assay for the detection of D2HG levels in various biological fluids
  • Supportive calculator (Click here to download a calculator excel file) Analyze results based on your experimental data!
  • Quick instruction bench card - to assure your experimental success
  • More experiments in one kit - contains sufficient reagents for 200 tests
  • Detection time: only 30-60 minutes

适用性

Suitable for the detection of D2HG levels in cells and tissues lysates, serum, urine, cultured cells and culture supernatants.

原理

Developed in partnership with the German Cancer Research Center (DKFZ), the D2HG Assay Kit is a rapid and sensitive enzymatic assay for the detection of D2HG levels in various biological fluids: serum, urine, cell culture supernatants, and cell or tissue lysates. The assay, originally developed by Balss et al., 8 is based on the oxidation of D2HG to α-ketoglutarate (αKG) by the enzyme (D)-2-hydroxyglutarate dehydrogenase (HGDH) coupled to the reduction of NAD+ to NADH (see Figure 1). The amount of NADH formed is then quantitated by the diaphorase mediated reduction of resazurin to the fluorescent dye resorufin (λex = 540 nm/λem = 590 nm).

法律信息

This product is sold under license from the German Cancer Research Center (DKFZ) and University Clinic of Heidelberg. Use of this product is covered by certain US and foreign patents, including US Pat. No. 9,487,815, EP Pat. No. 2 820 145 and foreign equivalents. Use of this product is for research purposes only.

产品性质

用途 sufficient for 200 reactions (Fluorometric)
detection methodfluorometric
运输wet ice
储存温度−20℃

安全信息

储存分类代码8A - Combustible, corrosive hazardous materials
WGKWGK 3

Sigma-Aldrich

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