产品说明
一般描述
The HIS-Select® Filter Plate is an immobilized metal-ion affinity chromatography (IMAC) product that allows rapid purification of small-scale crude cell extracts containing histidine-tagged proteins. The HIS-Select® Filter Plate contains 20 μm, spherical silica particles (100 nm pore size) with a hydrophilic layer.
The HIS-Select® Filter Plate is selective for recombinant proteins with histidine tags and exhibits very low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. The binding capacity of the HIS-Select® Filter Plate is typically > 2 mg per well as determined with an approximately 30 kDa histidine-tagged protein.
应用
The HIS-Select® Filter Plate is suitable for purification of recombinant proteins with histidine tags.
特点和优势
法律信息
Covered by US Patent No 6,623,655 and are sold for research use only. Commercial use requires additional licenses.
CelLytic is a trademark of sigma-aldrich Co. LLC
HIS-Select is a registered trademark of sigma-aldrich Co. LLC
基本信息
NACRES | NB.24 |
产品性质
等级 | for molecular biology |
形式 | plate |
储存温度 | 2-8℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H317 - H334 - H341 - H350i - H360D - H372 - H373 - H412 |
预防措施声明 | P202 - P260 - P273 - P280 - P302 + P352 - P308 + P313 |
危险分类 | Aquatic Chronic 3 - Carc. 1A Inhalation - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation |
靶器官 | Respiratory Tract |
储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich