产品说明
一般描述
IgG (immunoglobulin G) is part of the immunoglobulin family and is widely expressed. It possesses a γ heavy chain in the constant (C) region. IgG has a monomeric 150kDa structure, with two identical heavy chains and two identical light chains. They have a molecular weight of 50kDa and 25kDa, respectively. The primary structure of the antibody also contains disulfide bonds involved in linking the two heavy chains and the heavy and light chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion with papain cleaves the antibody into three fragments. Two of these fragments are identical and have antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains.
特异性
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Alkaline Phosphatase (calf intestine), Anti-Rabbit Serum, Cat IgG, Cat IgG F(ab′)2 and Cat Serum. No reaction was observed against Cat IgG F(c).
免疫原
Cat IgG F(ab′)2 fragment
物理属性
Antibody format: IgG
外形
Supplied in 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
基本信息
NACRES | NA.46 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
偶联物 | alkaline phosphatase conjugate |
抗体形式 | affinity isolated antibody |
antibody product type | secondary antibodies |
克隆 | polyclonal |
形式 | buffered aqueous solution |
species reactivity | cat |
浓度 | 0.5 mg/mL |
technique(s) | immunohistochemistry: suitable indirect ELISA: suitable western blot: suitable |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich