产品说明
一般描述
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs associate with rabbit IgGs.
免疫原
rabbit IgG F(ab′)2 fragment
应用
Atto 488-goat anti-rabbit IgG F(ab′)2 fragment, whole molecule antibody can be used for fluorescence-based immunoassays.
外形
Atto 488 F(ab)′2 fragment goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1mg/mL solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.
制备说明
F(ab)′2 fragment goat anti-rabbit is conjugated to Atto 488 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye.
分析说明
Unconjugated dye ≤5% of total fluorescence; dye-to protein ratio ≥2
法律信息
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.46 |
产品性质
偶联物 | Atto 488 conjugate |
质量水平 | 100 |
抗体形式 | F(ab′)2 fragment of affinity isolated antibody |
antibody product type | secondary antibodies |
克隆 | polyclonal |
包含 | 50% glycerol as stabilizer |
species reactivity | rabbit |
浓度 | 1 mg/mL protein |
荧光 | λex 500 nm; λem 522 nm in PBS |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves |
Sigma-Aldrich