产品说明
应用
Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.
法律信息
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
基本信息
| 经验(实验)分子式 | C37H53ClN6O8S |
| 分子量 | 777.37 |
| MDL编号 | MFCD04040671 |
| eCl@ss | 34058011 |
| PubChem化学物质编号 | 329747336 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 100 |
| 产品线 | BioReagent |
| 测定 | ≥95.0% (HPCE) |
| 形式 | powder |
| 溶解性 | DMF: soluble ethanol: soluble methanol: soluble |
| 荧光 | λex 520 nm; λem 540 nm in 0.1 M phosphate pH 7.0 |
| 适用性 | suitable for fluorescence |
| 储存温度 | 2-8℃ |
| SMILES string | [O-]Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=[NH+]\CC)\C(C)=CC3=C(CCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c2cc1C |
| InChI | 1S/C37H52N6O4S.ClHO4/c1-5-38-28-20-31-26(18-23(28)3)25(27-19-24(4)29(39-6-2)21-32(27)47-31)14-15-35(45)41-17-11-7-10-16-40-34(44)13-9-8-12-33-36-30(22-48-33)42-37(46)43-36;2-1(3,4)5/h18-21,30,33,36,38H,5-17,22H2,1-4H3,(H,40,44)(H,41,45)(H2,42,43,46);(H,2,3,4,5)/b39-29-; |
| InChI key | OCYRRKMPTKPPHX-QITHAKLPSA-N |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
