产品说明
一般描述
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry. Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150; aggregation number = 10) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
应用
CHAPS is suitable to prepare bicelles and to study the processes of liposome solubilization and reconstitution of transport proteins.
包装
20 mL Clear Glass Screw Cap Vial (850500P-1g)
基本信息
经验(实验)分子式 | C32H58N2O7S |
分子量 | 614.88 |
产品性质
测定 | >99% (TLC) |
形式 | powder |
分子量 | 614.88 g/mol |
包装 | pkg of 1 × 1 g (850500P-1g) |
manufacturer/tradename | Avanti Polar Lipids 850500P |
technique(s) | protein quantification: suitable |
运输 | dry ice |
储存温度 | −20℃ |
SMILES string | C[C@@]12C(CC[C@@H]2[C@@H](CCC(NCCC[N+](C)(CCCS(=O)([O-])=O)C)=O)C)C3[C@H](O)C[C@]4([H])C[C@H](O)CC[C@]4(C)C3C[C@@H]1O |
InChI | 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1 |
InChI key | UMCMPZBLKLEWAF-BCTGSCMUSA-N |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich