产品说明
一般描述
Collagen is the key structural protein of connective tissue and the most abundant protein in mammals. It occurs in many different types and forms with Types I -V being the most common. Aside from the crucial role it plays in the body, it has numerous medical applications such as its use in reconstructive surgery including bone and skin grafts. It is also commonly used in cosmetics due to its anti-aging and skin healing properties.
特点和优势
Compatible with high-throughput handling systems.
适用性
Suitable for biological samples (e.g. plasma, serum, cells) and cosmetic samples (e.g. collagen solution)
原理
Assay methods available for quantifying collagen currently range from needing extensive hydrolysis procedures with acids and bases to using expensive antibodies and complicated protocols. The collagen assay kit delivers a very simple, safe (non-radioactive), and sensitive method to quantify collagen in samples. In the first step of this procedure, collagen in the sample is enzymatically digested into peptides. Subsequently, the N-terminal glycine containing peptides react with the dye reagent to form a fluorescent complex. The fluorescence intensity of this product, measured at λex/em = 375/465 nm, is directly proportional to collagen concentration in the sample.
产品性质
用途 | sufficient for 100 fluorometric tests |
detection method | fluorometric |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H334 |
预防措施声明 | P261 - P284 - P501 |
危险分类 | Resp. Sens. 1 |
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
Sigma-Aldrich