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CompoZr® ZFA Targeting Human KLF4

品牌
Sigma-Aldrich
货号
CZFA1504
包装型号
规格纯度
参考价格
26103 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
CompoZr® ZFA Targeting Human KLF4
产品介绍:

产品说明

一般描述

Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted gene editing. Now, sigma-aldrich provides ZF activators, repressors and epigenetic modifiers for gene expression studies. ZFAs, or similarly called ZF−TFs (zinc finger transcription factors) incorporate a transcriptional activation domain in place of the nuclease domain found in ZFNs and specifically activate coding and non-coding genes. The increase in specific gene expression is dependent on the target binding site and the effector domain selected. Native gene activation is through ZFAs binding to promoters and recruiting the transcriptional machinery. This approach for activation also has the benefit of expressing endogenous isoforms at the proper ratio.
*Please note all ZFA plasmids are made standard with the p65 activation domain. The end-user will have to swap out the p65 domain and clone in the other activation, epigenetic or repressor domains as needed.

特点和优势

CompoZr ZFAs allows researchers to over-express endogenous genes with the advantage of having all splice variants in the appropriate ratios.

  • Validated ZFAs targeting KLF4 (human).
  • ZFA is a powerful, novel tool to modulate gene expression in human cells with low potential for off-targets.

组分

  • Validated ZFA plasmids with the p65 activation domain to upregulate human KLF4.

制备说明

Transcription Factor p65, or the RelA NF-kappaB (nuclear factor kappaB) subunit, contains a C-terminal transcriptional activation domain. Additional activation domains are included in the kit, which include plasmids with the 2xp65, VP16 and VP64 activation domains. 2xp65 consists of two repeating units of the p65 activation domain. VP16 is a trans-acting domain from the herpes simplex virus that induces early gene transcription. VP64 is a transactivating domain made up of a tetrameric repeat of VP16 minimal activation domain.

其他说明

If you are interested in the CompoZr ZFN technology for permanent editing of your gene of interest, please inquire about our Custom ZFN Service offering at: ZFNOps@sial.com

法律信息

CompoZr is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.51

产品性质

质量水平200
产品线CompoZr®
运输dry ice
储存温度−70℃

安全信息

WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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