您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > D-Lactate Assay Kit

D-Lactate Assay Kit

品牌
Sigma-Aldrich
货号
MAK336
规格纯度
sufficient for 100 colorimetric tests
参考价格
4962.3 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
D-Lactate Assay Kit
产品介绍:

产品说明

一般描述

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

特点和优势

Compatible with high-throughput handling systems.

适用性

Suitable for D-lactate in serum, plasma, and cell media samples.

原理

The D-Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.

产品性质

用途 sufficient for 100 colorimetric tests
detection methodcolorimetric
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

推荐产品