您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Duolink® PLA Multicolor Probemaker Kit-Red

Duolink® PLA Multicolor Probemaker Kit-Red

品牌
Sigma-Aldrich
货号
DUO96010
规格纯度
Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
参考价格
12296.7 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Duolink® PLA Multicolor Probemaker Kit-Red
产品介绍:

产品说明

应用

Specificity
Red fluorescence detection reagents are often used with Texas Red® filter.

Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells are required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list

特点和优势

Multicolor Duolink® proximity ligation assay (PLA®) is an application of the Duolink® technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink® PLA Probemaker Guide for Multicolor Detection and the Duolink® PLA Multicolor Detection protocol to use this product.

  • Visualize and detect multiple proteins events in one experiment.
  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Detection of low abundant proteins due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Faster and more simple than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Components
Duolink® PLA Multicolor Probemaker Kit - Red contains all the necessary reagents to perform the conjugations of Red Oligo A and Red Oligo B directly to primary antibodies. The resulting PLA probes (Red A and Red B) must be used together and cannot be paired with any other Duolink

This product is comprised of the following:
  • Red Oligo A DUO86010A
  • Red Oligo B DUO86010B
  • Conjugation Buffer DUO82033
  • Stop Reagent DUO82034
  • Storage Solution DUO82035

See datasheet for more information.

To perform a Multicolor Duolink® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink® Wash Buffers and Mounting Medium.

法律信息

Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC
Texas Red is a registered trademark of Life Technologies

产品性质

产品线Duolink®
technique(s)immunofluorescence: suitable
multiplexing: suitable
proximity ligation assay: suitable
荧光λex 594 nm; λem 624 nm (Texas Red®)
λex 594 nm; λem 624 nm (red)
适用性suitable for fluorescence
运输dry ice
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids

Sigma-Aldrich

推荐产品