产品说明
一般描述
The Extract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.
应用
Extract-N-Amp™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used for polymerase chain reaction (PCR).
特点和优势
原理
只需加入Extraction Solution并在室温下孵育5分钟,即可从10 μl全血中提取出基因组DNA。向提取物中加入Neutralization Solution,以便在PCR反应前中和抑制物。随后选取一部分DNA提取物,加入专门配制的PCR ReadyMix。
其他说明
更多信息,请见www.sigma-aldrich.com/extract-n-amp。
法律信息
凡购买本产品,即表示授权用户使用产品说明书所指定的专利,但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示,或通过禁止反言形式转让任何其他专利权(如,5′ Nuclease Process专利权)。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利,许可抗体用于体外研究用途。
Extract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
基本信息
NACRES | NA.55 |
产品性质
质量水平 | 200 |
用途 | sufficient for 1000 amplifications sufficient for 1000 extractions sufficient for 1000 reactions |
特点 | dNTPs included hotstart |
technique(s) | PCR: suitable |
颜色 | colorless |
web metadata keyword.default | XNAB2 XNAB2RE |
运输 | wet ice |
储存温度 | −20℃ |
组份列表
组份可单独销售
货号 | 组份 |
L3289 | Lysis Solution for Blood |
N9784 | Neutralization Solution for Blood |
P8115 | Extract-N-Amp™ PCR ReadyMix™ for Blood |
Sigma-Aldrich