产品说明
一般描述
The Human Amphiregulin (AR) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human AR in serum, plasma, cell culture supernatants and urine.
The AREG (amphiregulin) gene codes for a shedding ectodomain, derived from a transmembrane precursor of 252 amino acids. The encoded protein is released into blood or cellular vicinity through autocrine and paracrine. Areg is predominantly expressed in epithelial, mesenchymal cells and also by active immune cells. The AREG gene is mapped to human chromosome 4q13.3.
免疫原
Recombinant Human Amphiregulin
应用
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Amphiregulin ELISA Kit has been used to estimate the plasma concentration of amphiregulin by ELISA.
生化/生理作用
Areg (amphiregulin) stimulates growth-signaling pathways via EGF (epidermal growth factor) receptors. Areg participates in the organogenesis of mammary gland. It is regarded as a pro-oncogenic factor and contributes to tumor progression. Upregulation of the AREG gene is observed in cancer and resists chemotherapy. AREG reduces E-cadherin expression in order to promote invasion and progression of ovarian cancer.
其他说明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
基本信息
| NACRES | NA.32 |
产品性质
| species reactivity | human |
| 包装 | kit of 96 wells (12 strips x 8 wells) |
| technique(s) | ELISA: suitable capture ELISA: suitable |
| input | sample type serum sample type cell culture supernatant(s) sample type plasma sample type urine |
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 10 pg/mL standard curve range: 16.46-4000 pg/mL |
| detection method | colorimetric |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... AREG(374) |
组份列表
组份可单独销售
| 货号 | 组份 |
| RABELADA | ELISA 1X Assay/Sample Diluent Buffer A (Item D1) |
| RABELADB | ELISA 5X Assay/Sample Diluent Buffer B (Item E1) |
| RABSTOP3 | ELISA Stop Solution (Item I) |
| RABTMB3 | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) |
| RABWASH4 | 20X Wash Buffer (Item B) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H290 |
| 预防措施声明 | P234 - P390 |
| 危险分类 | Met. Corr. 1 |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
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