产品说明
一般描述
Cellular tumor antigen p53 is a tumor-suppressor protein that comprises transactivation at N-terminus, central sequence-specific DNA-binding domain, and regulatory domain at C-terminus. It plays a key role in autophagy regulation. It inhibits tumors and acts as a cellular stress sensor especially during nutrient deprivation, hypoxia, and ribosome dysfunction. The Human p-53 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human p-53 in serum, plasma, cell culture supernatants and urine.
免疫原
Recombinant Human p53
应用
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human p53 ELISA Kit has been used for quantifying p53 using enzyme-linked immunosorbent assay (ELISA) in human myeloma RPMI-8226 cells, human colorectal cancer HCT-116 cells, human breast cancer MCF-7 cells and human leukemiaHL-60 cells.
其他说明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
基本信息
| NACRES | NA.32 |
产品性质
| species reactivity | human |
| 包装 | kit of 96 wells (12 strips x 8 wells) |
| technique(s) | ELISA: suitable capture ELISA: suitable |
| input | sample type urine sample type serum sample type plasma sample type cell culture supernatant(s) |
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 0.3 ng/mL standard curve range: 0.328-80 ng/mL |
| detection method | colorimetric |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... TP53(7157) |
组份列表
组份可单独销售
| 货号 | 组份 |
| RABELADB | ELISA 5X Assay/Sample Diluent Buffer B (Item E1) |
| RABELADD | ELISA 5X Assay/Sample Diluent Buffer D (Item K) |
| RABSTOP3 | ELISA Stop Solution (Item I) |
| RABTMB3 | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) |
| RABWASH4 | 20X Wash Buffer (Item B) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H290 |
| 预防措施声明 | P234 - P390 |
| 危险分类 | Met. Corr. 1 |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
Sigma-Aldrich
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