产品说明
一般描述
The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.
应用
In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.
生化/生理作用
染料与细胞蛋白结合并随后溶解在碱中。
基本信息
NACRES | NA.84 |
产品性质
形式 | liquid |
质量水平 | 200 |
用途 | 1 kit sufficient for 1,000 tests |
包装 | pkg of 1 kit |
储存条件 | dry at room temperature |
λmax | 565 nm |
web metadata keyword.default | cytotoxicity assay substrate, colorimetric determination, 565 nM detection |
application(s) | cell analysis detection |
detection method | colorimetric |
储存温度 | room temp |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H314 - H335 - H410 |
预防措施声明 | P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338 + P310 |
危险分类 | Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3 |
靶器官 | Respiratory system |
储存分类代码 | 8A - Combustible, corrosive hazardous materials |
Sigma-Aldrich