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Mouse CRISPR Brie Knockout Library

品牌
Sigma-Aldrich
货号
PCRISPR004
包装型号
规格纯度
参考价格
71167.4 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Mouse CRISPR Brie Knockout Library
产品介绍:

产品说明

一般描述

The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

应用

  • Functional Genomics/Target Validation
  • Unbiased wholed genome forward genetic screening
  • Validated positive and negative controls
  • Set up and optimization of screen assay

特点和优势

  • Focus on your research, and we will generate your lentivirus screening library.
  • Use CRISPR nucleases to knockout protein-coding genes to assess their function.
  • Mouse Genes targeted 19,674
  • Compact library of ~ four gRNAs per gene (78,637 total)
  • Total Controls 1000(pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO for sources of Cas9.

原理

In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.

法律信息

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

产品性质

包装pkg of 5 vials (5x200µL aliquots )
浓度≥5x108 VP/ml (via p24 Assay)
application(s)CRISPR
运输dry ice
储存温度−70℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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