产品说明

一般描述

Purine Nucleoside Phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allows the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP deficiency leads to severe combined immunodeficiency and also leads to hypouricemia and reduced urinary excretion of uric acid.

适用性

Suitable for the detection of Purine Nucleoside Phosphorylase activity in cell and tissue lysate or purified recombinant protein

原理

In this Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed from the breakdown of inosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the PNP Probe. The fluorescent product is measured at λex = 535nm/λem = 587nm. Limit of quantification is 0.005 μU of recombinant purine nucleoside phosphorylase.

基本信息

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H290 - H314 - H334 - H412
预防措施声明	P234 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
危险分类	Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B
储存分类代码	8A - Combustible, corrosive hazardous materials
WGK	WGK 3