产品说明
一般描述
SW48 Cells HIF1A -/-/- are colorectal adenocarinoma, epithelial cells, from a human adult female, with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-231.
应用
Media Renenewal changes one to two times per week.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture ratio: approx. 1:2 to 1:6.
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Cat. No. S8636, to a final concentration of 1 mM.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
特点和优势
These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2
质量
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
基本信息
NACRES | NA.81 |
产品性质
质量水平 | 200 |
生物来源 | human female colon (Source disease: Colorectal adenocarcinoma) |
储存温度 | −196℃ |
Gene Information | human ... HIF1A(3091) |
组份列表
组份可单独销售
货号 | 组份 |
SW48 CELLS HIF1A -/-/- 1 vial |
Sigma-Aldrich