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SW48 Cells PTEN -/-

品牌
Sigma-Aldrich
货号
CLLS1009
包装型号
规格纯度
参考价格
59056.1 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
SW48 Cells PTEN -/-
产品介绍:

产品说明

一般描述

SW48 Cells PTEN -/- are colorectal adenocarcinoma, epithelial cells, from a human caucasian female (aged 82 years), with a ZFN knock out modification.

应用

Media Renewal changes one to two times per week
The cell freezing medium is DMSO 1X (Cat. No. C6164)
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
Subculture Ratio: approx. 1:2 to 1:6
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.

特点和优势

These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 10q23.3

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

基本信息

NACRESNA.81

产品性质

质量水平200
生物来源human female colon (Source Disease: Colorectal adenocarcinoma)
OMIM登记号601728
储存温度−196℃
Gene Informationhuman ... PTEN(5728)

组份列表

组份可单独销售

货号组份
SW48 Cells PTEN -/- 1 vial
SW48 Control Cells 1 vial

Sigma-Aldrich

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