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Thioredoxin Reductase Kit

品牌
Sigma-Aldrich
货号
MAK409
规格纯度
sufficient for 100 colorimetric tests
参考价格
5736.34 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Thioredoxin Reductase Kit
产品介绍:

产品说明

一般描述

Thioredoxin reductase (TrxR) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.

特点和优势

Thioredoxin Reductase Kit:

  • Simple and fast procedure with results in ∼40 minutes.

适用性

The kit is suitable for the measurement of thioredoxin reductase (TrxR) in various samples including tissue, cells and serum.

原理

In the assay TrxR catalyzes the reduction of 5, 5′-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generates a strong yellow color at A412 nm. In crude biological samples, other enzymes such as glutathione reductase and glutathione peroxidase can also reduce DTNB. Therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed. The first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.

产品性质

质量水平100
web metadata keyword.defaultThioredoxin Reductase Kit|sigma-aldrich
detection methodcolorimetric
储存温度−20℃

安全信息

象形图GHS05
警示用语:Danger
危险声明H315 - H318
预防措施声明P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
危险分类Eye Dam. 1 - Skin Irrit. 2
储存分类代码10 - Combustible liquids

Sigma-Aldrich

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