产品说明
一般描述
Thioredoxin reductase (TrxR) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.
特点和优势
Thioredoxin Reductase Kit:
适用性
The kit is suitable for the measurement of thioredoxin reductase (TrxR) in various samples including tissue, cells and serum.
原理
In the assay TrxR catalyzes the reduction of 5, 5′-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generates a strong yellow color at A412 nm. In crude biological samples, other enzymes such as glutathione reductase and glutathione peroxidase can also reduce DTNB. Therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed. The first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.
产品性质
质量水平 | 100 |
web metadata keyword.default | Thioredoxin Reductase Kit|sigma-aldrich |
detection method | colorimetric |
储存温度 | −20℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H315 - H318 |
预防措施声明 | P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364 |
危险分类 | Eye Dam. 1 - Skin Irrit. 2 |
储存分类代码 | 10 - Combustible liquids |
Sigma-Aldrich