Tyrosinase Activity Assay Kit (Colorimetric)

产品说明

一般描述

Tyrosinase is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway; first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin. Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors, therefore, is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific diagnostic test for melanoma and may be useful in monitoring patient response to melanoma treatments.

特点和优势

Tyrosinase Activity Assay Kit:

• Non-radioactive assay.
• Sensitive, detecting Tyrosinase Activity ≤30 μU.

适用性

The kit is suitable for the measurement of tyrosinase activity in cell lysates (e.g. Melanoma cells), tissue lysates, plant tissue lysates (e.g. potato), recombinant enzyme and purified protein.

原理

In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at A₅₁₀ nm. The assay can detect as low as 30 μU Tyrosinase in biological samples.

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H301 - H315 - H319 - H335
预防措施声明	P261 - P264 - P270 - P301 + P310 - P302 + P352 - P305 + P351 + P338
危险分类	Acute Tox. 3 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官	Respiratory system
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects