产品说明
一般描述
Adenosyl homocysteinase (AHCY) (EC 3.3.1.1) or S-adenosyl homocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies).
适用性
Suitable for screening, characterizing and studying AHCY inhibitors
原理
In this AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence (λex = 392 nm/λem = 482 nm). The AHCY reaction is reversible and hence adenosine deaminase is included in the reaction to ensure the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.
基本信息
| NACRES | NA.25 |
产品性质
| 质量水平 | 100 |
| 用途 | sufficient for 100 fluorometric tests |
| detection method | fluorometric |
| 储存温度 | −20℃ |
安全信息
| 象形图 |    |
| 警示用语： | Danger |
| 危险声明 | H312 - H317 - H318 - H334 - H361d - H412 |
| 预防措施声明 | P202 - P273 - P280 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313 |
| 危险分类 | Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Sens. 1 |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
