Alkaline Phosphatase Activity Fluorometric Assay Kit

产品说明

一般描述

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. The change in alkaline phosphatase level and activity is associated with several diseases in the liver and bones. Alkaline phosphatase is also a popular enzyme conjugated to secondary antibody in ELISA assays.

特点和优势

Alkaline Phosphatase Activity Fluorometric Assay Kit:

• Compatible with high-throughput handling systems.
• Simple procedure with results in ∼1 hour.
• Ultra-sensitive, with a detection sensitivity of ∼1 μU.

适用性

The kit is suitable for the measurement of alkaline phosphatase activity in serum, plasma, urine, semen, cell culture media, cells and tissue.

原理

In the Alkaline Phosphatase Activity Fluorometric Assay Kit, ALP cleaves the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (MUP) substrate resulting in an intense fluorescent signal (λ_Ex = 360 nm/λ_Em = 440 nm).

产品性质

安全信息