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Anti- Bovine IgG-Peroxidase antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200796
规格纯度
clone BG-18, purified from hybridoma cell culture
参考价格
6513.63 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti- Bovine IgG-Peroxidase antibody, Mouse monoclonal
Bovine immunoglobulin G
产品介绍:

产品说明

一般描述

Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the BG-18 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with the purified bovine IgG.
Immunoglobulin G (IgG) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.
The bovine immunoglobulin system closely resembles that of other mammalian species with respect to the physiochemical properties and nomenclature. The main bovine IgG class antibodies are IgG1 and IgG2, which have antigenic differences in the Fc region of the heavy chains.4 

特异性

Monoclonal Anti-Bovine IgG specifically recognizes an epitope on the heavy chain of both bovine IgG1 and IgG2. The antibody shows no cross-reactivity with bovine IgM or IgG from the following species: human, goat, sheep, rabbit, pig, dog, chicken, cat, guinea pig or horse.

免疫原

Purified bovine IgG

应用

The antibody is recommended to use in various immunological techniques, including ELISA1, Immunohistochemistry2 and Immunobloting3

生化/生理作用

Different immunoglobulin classes and subclasses (isotypes) perform distinctive effector functions. Therefore, the ability to characterize antibody isotype is fundamental to the analysis of the immune response. The diversity of immunoglobulin isotypes associated with the immunity pattern serves as an indicator indistinguishing the phase of infection with various agents.

外形

Supplied as a lyophilized powder.

储存及稳定性

Store the lyophilized product at 2–8 ℃. For extended storage after reconstitution, keep at –20 ℃ in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 ℃ for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源mouse
偶联物peroxidase conjugate
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆BG-18, monoclonal
形式lyophilized powder
species reactivitybovine
浓度~2 mg/mL
technique(s)direct ELISA: 1:20,000-1:40,000 using 1 μg/ml bovine IgG for coating
同位素/亚型IgG1
运输dry ice
储存温度−20℃

安全信息

象形图GHS07
警示用语:Warning
危险声明H317
预防措施声明P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
危险分类Skin Sens. 1
储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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