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Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit

品牌
Sigma-Aldrich
货号
A2418
规格纯度
IgG fraction of antiserum, buffered aqueous solution
参考价格
5146.79 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit
产品介绍:

产品说明

一般描述

IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.

免疫原

purified mouse IgG

应用

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in :

  • direct enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunohistochemistry

Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.

生化/生理作用

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG antibody participates in complement fixation and opsonization.

外形

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162640

产品性质

生物来源rabbit
质量水平200
偶联物alkaline phosphatase conjugate
抗体形式IgG fraction of antiserum
antibody product typesecondary antibodies
克隆polyclonal
形式buffered aqueous solution
species reactivitymouse
technique(s)direct ELISA: 1:15,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil tissue
western blot (chemiluminescent): 1:40,000-1:80,000 using deecting β-Actin in total cell extract of HeLa cells (5-10 μg/mL)
运输wet ice
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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