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Anti-Rat IgG (whole molecule)-Peroxidase antibody produced in rabbit

抗鼠 IgG(全分子)- 过氧化物酶 兔抗

品牌
Sigma-Aldrich
货号
A9542
规格纯度
IgG fraction of antiserum, buffered aqueous solution
参考价格
5112.7 *本价格含增值税费
促销
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数量
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产品名称:
Anti-Rat IgG (whole molecule)-Peroxidase antibody produced in rabbit
抗鼠 IgG(全分子)- 过氧化物酶 兔抗
产品介绍:

产品说明

一般描述

Immunoglobulins have two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein which mainly helps in immune defense. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
IgG (Immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

特异性

Anti-Rat IgG (whole molecule) antibody recognizes rat (immunoglobulin G) IgG and is determined by immunoelectrophoresis (IEP) versus normal rat serum and rat IgG prior to conjugation.

免疫原

purified rat IgG

应用

Anti-Rat IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in western blotting and enzyme linked immunosorbent assay (ELISA).

生化/生理作用

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

制备说明

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162813

产品性质

生物来源rabbit
质量水平200
偶联物peroxidase conjugate
抗体形式IgG fraction of antiserum
antibody product typesecondary antibodies
克隆polyclonal
形式buffered aqueous solution
species reactivityrat
technique(s)direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500
western blot (chemiluminescent): 1:5,000 using detecting rat primary antibody
运输dry ice
储存温度−20℃

安全信息

象形图GHS08
警示用语:Danger
危险声明H317 - H334
预防措施声明P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
危险分类Resp. Sens. 1 - Skin Sens. 1
储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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