产品说明
一般描述
Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose.
特异性
该抗体可识别包含VSV糖蛋白的五个羧基末端氨基酸的表位。在受感染的细胞中,该抗体将未成熟型VSV-G定位在粗糙内质网(RER)和高尔基复合体潴泡中,将成熟型VSV-G定位在细胞表面和出芽病毒中。该抗体不会染色缺少膜和细胞质结构域的分泌型VSV-G。该抗体可用于微注射抗体、免疫印迹、免疫沉淀、免疫细胞化学和免疫电镜法研究新合成的VSV-G在向质膜和细胞表面转移过程中细胞质结构域的作用。该抗体可用于对以VSV-G羧基末端标记的外源性导入结构进行检测、免疫沉淀和免疫细胞化学染色。该标记不会干扰所研究蛋白质的功能,可被P5D4抗体特异性识别,而不与任何内源性蛋白质发生交叉反应。
免疫原
KLH标记的含有水泡性口炎病毒糖蛋白(VSV-G)的15个羧基末端氨基酸(497-511)的合成肽。
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.
外形
Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
制备说明
Prepared using cyanogen bromide-activated agarose.
基本信息
NACRES | NA.56 |
产品性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | agarose conjugate |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | P5D4, monoclonal |
形式 | PBS suspension |
analyte chemical class(es) | proteins (VSV-G) |
technique(s) | immunoprecipitation (IP): suitable protein purification: suitable |
同位素/亚型 | IgG1 |
容量 | ≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein) |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich