产品说明
一般描述
Cell cycle is a ubiquitous, complex sequence of events leading to growth and proliferation of cells. Cell cycle progression is tightly regulated due to its involvement in development, DNA damage and repair, etc. Anomalies in cell cycle progression can lead to tissue hyperplasia and diseases such as cancer. Cell cycle can be subdivided into interphase (G0/G1, S and G2) and mitotic (M) phase (prophase, metaphase, anaphase and telophase).
特点和优势
The method is non-radioactive, rapid and accurate and can be used for high throughput cell cycle analysis with contemporary flow cytometer instruments.
适用性
Suitable for studying cell cycle in adherent or suspension cells
原理
The kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content. The percentages of cells in different phases of the cell cycle (G0/G1, S, and G2/M) can be quantified by flow cytometry.
产品性质
用途 | sufficient for 100 assays |
detection method | fluorometric |
储存温度 | −20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich