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位置:首页 > 品牌 > Sigma-Aldrich > Duolink® In Situ Orange Starter Kit Mouse/Goat

Duolink® In Situ Orange Starter Kit Mouse/Goat

品牌
Sigma-Aldrich
货号
DUO92104
包装型号
规格纯度
参考价格
20370.5 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Duolink® In Situ Orange Starter Kit Mouse/Goat
产品介绍:

产品说明

应用

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink® In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list

特点和优势

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

法律信息

Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC

产品性质

质量水平100
产品线Duolink®
technique(s)proximity ligation assay: suitable
荧光λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)
适用性suitable for fluorescence

组份列表

组份可单独销售

货号组份
DUO92006Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)
DUO92001Duolink® In Situ PLA® Probe Anti-Mouse PLUS
DUO92007Duolink® In Situ Detection Reagents Orange
DUO82049Duolink® In Situ Wash Buffers, Fluorescence
DUO82040Duolink® In Situ Mounting Medium with DAPI

安全信息

象形图GHS05GHS07GHS09
警示用语:Warning
危险声明H290 - H317 - H411
预防措施声明P234 - P261 - P272 - P273 - P280 - P302 + P352
危险分类Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1
储存分类代码8A - Combustible, corrosive hazardous materials
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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