Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)

产品说明

一般描述

Gelatinases are a type of matrix zinc-dependent metalloproteases (MMPs) that degrade gelatins and a variety of other extracellular matrix proteins. These enzymes are synthesized as latent zymogens that are activated by proteolysis and inhibited by tissue inhibitors of metalloproteases (TIMPs). Two mammalian gelatinases, Gelatinase A (MMP-2) and Gelatinase B (MMP-9), are critical for basement membrane degradation and are highly upregulated in variety of tumor cells.

特点和优势

Compatible with high-throughput handling systems.

适用性

Suitable for the detection of gelatinase activity in biological samples such as recombinant protein, tissue, cell lysates, etc

原理

The Gelatinase Activity Assay Kit utilizes a hybrid approach for the detection of gelatinase activity by employing a highly quenched gelatin substrate which upon cleavage by a suitable gelatinase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of gelatinase activity (as low as 0.06 mCDU).

产品性质

安全信息