产品说明
一般描述
Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters.
适用性
焦磷酸检测试剂盒提供了一种简单、直接的荧光微孔板检测方法,适于检测多种样本中的焦磷酸水平。
原理
The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =370/λem = 470 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.
基本信息
EC 号 | 200-664-3 |
NACRES | NA.84 |
产品性质
用途 | sufficient for 200 fluorometric tests |
detection method | fluorometric |
储存温度 | −20℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H318 |
预防措施声明 | P280 - P305 + P351 + P338 |
危险分类 | Eye Dam. 1 |
储存分类代码 | 10 - Combustible liquids |
Sigma-Aldrich